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Image Search Results
Journal: Cell Death & Disease
Article Title: Novel prosurvival function of Yip1A in human cervical cancer cells: constitutive activation of the IRE1 and PERK pathways of the unfolded protein response
doi: 10.1038/cddis.2017.147
Figure Lengend Snippet: Depletion of Yip1A induces apoptotic cell death in HeLa and CaSki cervical cancer cells. ( a ) HeLa and CaSki cells were transfected with control scramble siRNA (left panel) or Yip1A siRNA (right panel). Representative confocal micrographs show morphological differences between control and Yip1A-knockdown cells at the indicated time points. Scale bars are 50 μ m. ( b ) The viability of transfected cells was evaluated at the indicated time points using the MTT Cell Proliferation Assay. Data are means±S.D. from three independent experiments; * P <0.05, ** P <0.01. ( c ) Representative flow cytometric data for control (left panels) and Yip1A-knockdown (right panels) cells at the indicated time points after siRNA transfection. The percentages of apoptotic cells (Annexin V + /PI − + Annexin V + /PI + ) are shown in the bar graphs. Data are means±S.D. from three independent experiments; * P <0.05. ( d ) Representative confocal micrographs of control (upper panels) and Yip1A-knockdown (lower panels) cells at the indicated time points after siRNA transfection. Scale bars are 10 μ m. The percentages of TUNEL-positive cells are shown in the bar graphs. Data are means±S.D. from three independent experiments; * P <0.05, ** P <0.01. ( e ) Western blotting shows relative levels of cleaved caspase 3 and cleaved PARP protein in control and Yip1A-knockdown cells at the indicated time points after siRNA transfection. GAPDH was used for normalization. Data are means±S.D. from three independent experiments; * P <0.05, ** P <0.01
Article Snippet: A human
Techniques: Transfection, Control, Knockdown, MTT Cell Proliferation, TUNEL Assay, Western Blot
Journal: Cell Death & Disease
Article Title: Novel prosurvival function of Yip1A in human cervical cancer cells: constitutive activation of the IRE1 and PERK pathways of the unfolded protein response
doi: 10.1038/cddis.2017.147
Figure Lengend Snippet: Schematic representation of how Yip1A operates as a prosurvival modulator that coordinately activates the IRE1 and PERK pathways of the UPR to support the survival of HeLa and CaSki cervical cancer cells. See text for details. Ub, ubiquitination
Article Snippet: A human
Techniques: Ubiquitin Proteomics